徐贵发 赵秀兰 赵 丽 胡 萍 孙淑爱 徐贵发 赵秀兰 赵 丽 胡 萍 孙淑爱 山东医科大学营养与食品卫生教研室，济南 250012 卫生研究 1999 0 28 3


    关键词：麦胚；黄酮类化合物；细胞培养；生长抑制 期刊 wsyj 0 达能营养中心青年科学工作者论坛 fur -->


    

摘要： 应用MTT法、克隆形成实验、³ H-TdR掺入实验测定了麦胚黄酮类提取物对人乳腺髓样癌细胞株BCap-37的生长抑制作用，结果显示麦胚黄酮类提取物能明显抑制人乳腺髓样癌细胞株BCap-37的生长、克隆形成和DNA合成能力，均呈现明显的剂量-效应关系，并随作用时间延长其效果增强；因此麦胚黄酮类提取物可能是通过抑制了BCap-37细胞DNA的合成而降低其生长和繁殖的能力。

    中图分类号： R737.9 R730.51 O625.31

Inhibition of human breast cancer cell line BCap-37

    by flavonoid extract of wheat germ in vitro

Xu Guifa, Zhao Xiulan, Zhao Li,Xu HuPing,et al.

    Department of Nutrition and food hygiene,Shandong Medical University,Jinan 250012,China

Cell growth and proliferation were measured by microculture tetrazolium(MTT) assay,cell colony-forming assay and the synthesis of DNA by ³ H-thymidine incorporation. Flavonoid extract of wheat germ resulted to a dose-dependent,time-dependent growth inhibition,reduction of colony and ³ H-thymidingincorporation in DNA of human breast cancer cell BCap-37.These findings indicated that theflavonoid extract of wheat germ can inhibit tumor cell growth and proliferation byblocking DNA synthesis in vitro.

     ......